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US 12,086,722 B2Granted
DNA-based digital information storage with sidewall electrodes
AssigneeTwist Bioscience Corporation
InventorsBrian Wayne Bramlett, Bill James Peck
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Abstract
Provided herein are compositions, devices, systems and methods for generation and use of biomolecule-based information for storage. Further provided are devices-having addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.) The compositions, devices, systems and methods described herein provide improved storage, density, and retrieval of biomolecule-based information.
Claims20
A method for storing nucleic acid-based digital information, comprising:
- a) providing a device comprising:
- a solid support, wherein the solid support comprises a plurality of wells, wherein each of the wells comprises an addressable locus comprising:
- a synthesis surface located in a bottom region of each of the wells;
- a bottom electrode in addressable communication with the synthesis surface;
- and
- at least one sidewall electrode located on a sidewall of each of the wells;
- b) depositing at least one blocked nucleoside on the synthesis surface, wherein the at least one blocked nucleoside couples to a polynucleotide attached to the synthesis surface;
- and
- c) deblocking the at least one blocked nucleoside on the synthesis surface, wherein deblocking comprises applying an electrical potential to the at least one sidewall electrode closest to the blocked nucleoside relative to the bottom electrode or a different sidewall electrode, and
- d) repeating steps b) and c) to synthesize a plurality of polynucleotides on the synthesis surface, wherein at least some of the polynucleotides encode for digital information.
7 more elements
Dependencies are inferred from the claim text.