Microscopic image capturing method and microscopic image capturing device

A microscopic image capturing method for capturing microscopic image using a microscopic image capturing device, wherein

• the microscopic image is a microscopic image of a cell or a particle as a sample in contact with an inner side of a bottom surface of a container,
• the microscopic image capturing device includes
• a transparent container configured to accommodate a sample,
• a light source for microscope imaging,
• a light beam light source configured to emit a light beam toward the inner side of the bottom surface of the container,
• an objective lens used to form a spot image of the light beam reflected by the sample or the container,
• a detector configured to detect the formed spot image, and
• a moving mechanism configured to relatively move the container and the objective lens, and
• in the method, the microscopic image capturing device further includes a determination unit configured to determine whether the light beam has been applied to a defect based on the spot image,
• the microscopic image capturing method comprising:
• a step of emitting the light beam from the light beam light source;
• a step of detecting the spot image by the detector;
• a step of focusing the spot image by the moving mechanism moving the objective lens in an optical axis direction of the light beam with respect to the container;
• a step of determining by the determination unit whether the light beam has been applied to a defect based on the spot image,
• a step a) of, when it is determined that the light beam has been applied to the defect, moving by the moving mechanism the container with respect to the objective lens in a direction orthogonal to an optical axis of the light beam according to a prescribed condition;
• and
• a step b) of, when it is determined that the light beam has not been applied to the defect, capturing a microscopic image of the sample using the light source for microscope imaging.