Back to results
US 12,084,698 B2Granted
Virus-like particles and use thereof
AssigneeKyoto University
InventorsAkitsu Hotta, Peter David Gee
Life of this patent
Estimating the term…
Abstract
A virus-like particle encapsulating a target protein is provided. The virus-like particle contain a Gag protein, and the Gag protein forms a dimer with the target protein.
Claims6
A virus-like particle encapsulating a Cas family protein and a gRNA, comprising:
- a fusion protein of FKBP12 and Gag protein and a fusion protein of FRB and the Cas family protein, or a fusion protein of FRB and Gag protein and a fusion protein of FKBP12 and a Cas family protein;
- an mRNA or a self-cleavage product of the mRNA wherein the mRNA having the gRNA sequence interposed between a first ribozyme sequence and a second ribozyme sequence and a packaging signal sequence;
- and
- a rapamycin or a rapamycin derivative;
- wherein the FKBP12, the rapamycin or the rapamycin derivative, and the FRB are bound together and the Gag protein forms a dimer with the Cas family protein.
2 more elements
A method for manufacturing a virus-like particle encapsulating a Cas family protein and a gRNA, comprising the following (1) and (2):
- (1) letting a cell to express:
- a fusion protein of FKBP12 and Gag protein and a fusion protein of FRB and the Cas family protein, or a fusion protein of FRB and Gag protein and a fusion protein of FKBP12 and a Cas family protein, and
- an mRNA having the gRNA sequence interposed between a first ribozyme sequence and a second ribozyme sequence and a packaging signal sequence in the presence of rapamycin or a rapamycin derivative;
- and
- (2) obtaining a medium containing the virus-like particle encapsulating the Cas family protein and the gRNA.
2 more elements
Dependencies are inferred from the claim text.