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US 12,084,681 B2Granted
Ex vivo antibody production
AssigneeKling Biotherapeutics B.V.
InventorsPaula Maria Wilhelmina Van Helden, Mark Jeroen Kwakkenbos, Hergen Spits, Tim Beaumont
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Abstract
The present invention provides means and methods for producing improved ex vivo B cell cultures with a short doubling time.
Claims19
A method for obtaining antibodies comprising:
- inducing, enhancing and/or maintaining expression of Bcl-6, or a rabbit homologue thereof, in a rabbit B cell;
- inducing, enhancing and/or maintaining expression of at least one anti-apoptotic nucleic acid molecule in said B cell,
- culturing said B cell ex vivo to produce antibodies in a culture medium;
- and
- harvesting antibodies from the culture medium in an amount of at least 100 ng/ml culture medium within 10-14 days from the start of the culturing.
2 more elements
Dependencies are inferred from the claim text.