Packaging oligonucleotides into virus-like particles

A process for producing a nucleotide composition comprising aggregated oligonucleotides, said process comprising the steps of:

• (a) providing oligonucleotides which comprise 3 to 15 guanosine entities at the 5′ end and 3 to 15 guanosine entities at the 3′ end, and wherein said oligonucleotides comprise phosphodiester connected deoxynucleotides;
• (b) denaturing said oligonucleotides, wherein said denaturing comprises the step of
• (i) incubating an aqueous solution I comprising said oligonucleotides and a chaotropic agent at 75° C. to 99° C. until the average diameter of said oligonucleotides is 1 nm or less, wherein said average diameter is determined by Dynamic Light Scattering (DLS),
• (c) aggregating said oligonucleotides, wherein said aggregating comprises the steps of
• (i) incubating an aqueous solution II comprising said oligonucleotides having said average diameter of 1 nm or less obtained in step (b), a chaotropic agent and a cation at 75° C. to 99° C. to form said aggregated oligonucleotides, wherein said incubating is performed until the average diameter of said formed aggregated oligonucleotides is 7-14 nm, wherein said average diameter is determined by Dynamic Light Scattering (DLS),
• (ii) adjusting the temperature of said solution II to below 40° C.