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US 12,084,650 B2Granted
Preservation of cell-free nucleic acids in biological samples
AssigneeNorgen Biotek Corp.
InventorsTaha Alexander Haj-Ahmad, Yousef Haj-Ahmad
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Abstract
Provided is a composition for preserving cell-free nucleic acids and/or cells in a biological sample and methods for use thereof. The composition comprises at least one volume excluding polymer, at least one osmotic agent and at least one enzyme inhibitor. The composition optionally further comprises at least one metabolic inhibitor. Further, provided is a kit comprising the composition, preferably in a blood collection tube, or the components of the composition.
Claims13
A method for preserving nucleic acids and/or cells in a biological sample comprising the steps of:
- providing a composition comprising:
- at least one volume excluding polymer, wherein the at least one volume excluding polymer is polyethylene glycol (PEG) present in an amount of about 20 to about 40% by weight of the composition;
- at least one osmotic agent, wherein the at least one osmotic agent is NaCl present in an amount of about 1 to about 20% by weight of the composition;
- at least one enzyme inhibitor, wherein the at least one enzyme inhibitor is EDTA or citrate present in an amount from about 1 to about 30% by weight of the composition;
- and
- a metabolic inhibitor, wherein the metabolic inhibitor is sodium azide present in an amount from about 0.01 to about 10% by weight of the composition;
- and
- preventing or reducing the degradation of nucleic acids and/or cell lysis in the biological sample by contacting the biological sample with the composition to provide a treated sample.
5 more elements
Dependencies are inferred from the claim text.