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US 12,083,499 B2Granted
Guard column and method for producing guard column
AssigneeResonac Corporation
InventorsHirotsugu Nakanishi, Ritsuko Wakayama
Life of this patent
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Abstract
Provided is a guard column including a filling part having a length of 2.0 cm to 3.5 cm formed of a filler, in which the filler is made of porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm, and a pressure difference when an aqueous solution is fed at a linear flow rate of 2.1 cm/min is 4.0 MPa or more.
Claims14
A guard column, comprising
- a filling part having a length of 2.0 cm to 3.5 cm formed of a filler, wherein
- the filler includes a porous silica gel,
- the porous silica gel has a hydrophilized surface, an average particle size of 1.5 μm to 2.5 μm, an average pore size of 25 nm to 35 nm, and a specific surface area of 100 m2/g to 300 m2/g, and
- a pressure difference when an aqueous solution is fed to the guard column at a linear flow rate of 2.1 cm/min is 4.0 MPa or more.
1 more element
A guard column, comprising a filling part having a length of 2.0 cm to 3.5 cm formed of a filler, wherein
- the filler includes a porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm,
- a pressure difference when an aqueous solution is fed at a linear flow rate of 2.1 cm/min is 4.0 MPa or more, and
- the guard column is used for size-exclusion chromatography in which a column using a filler containing porous silica gel and a light scattering detector are used with the guard column.
A guard column, comprising a filling part having a length of 2.0 cm to 3.5 cm formed of a filler, wherein
- the filler includes a porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm,
- a pressure difference when an aqueous solution is fed at a linear flow rate of 2.1 cm/min is 4.0 MPa or more, and
- wherein the porous silica gel having a hydrophilized surface is a silica gel which is obtained by treating porous silica gel, whose surface is not hydrophilized, with a silane coupling agent to form a functional group derived from the silane coupling agent and further reacting the functional group with a hydrophilic compound.
Dependencies are inferred from the claim text.