Method for purifying cannabinoids
AssigneeSartorius Chromatography Equipment
InventorJin Seok Hur
Life of this patent
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Abstract
The invention relates to a method for the chromatographic purification of at least one cannabinoid compound, wherein the method comprises a main purification stage comprising the steps of: injecting an initial mixture comprising the at least one cannabinoid compound and one or more additional compounds onto a main stationary phase comprising silica particles, the silica particles comprising amino and/or diol groups; performing an elution with an elution solution, and collecting one or more elution fractions; and optionally, washing the main stationary phase with a washing solution and collecting one or more washing fractions; at least one of the elution fractions or washing fractions containing the at least one cannabinoid compound purified from the one or more additional compounds.
Claims19
A method for the chromatographic purification of at least one cannabinoid compound, wherein the method comprises a main purification stage comprising the steps of:
- injecting an initial mixture comprising the at least one cannabinoid compound and one or more additional compounds onto a main stationary phase comprising silica particles in a chromatographic installation having a non-static bed;
- performing an elution with an elution solution, and collecting one or more elution fractions;
- and
- optionally, washing the main stationary phase with a washing solution and collecting one or more washing fractions;
- wherein
- at least one of the elution fractions or washing fractions contains the at least one cannabinoid compound purified from the one or more additional compounds,
- the elution solution is a mixture of a polar solvent and a non-polar solvent, the polar solvent being an alcohol and the non-polar solvent being an alkane, and
- the elution is performed in an isocratic mode, and
- the method further comprises a preliminary purification stage before the main purification stage, said preliminary purification stage comprising the steps of:
- putting a preliminary mixture comprising the at least one cannabinoid compound, the additional compounds and one or more further compounds in contact with a preliminary stationary phase;
- and
- collecting at least one liquid fraction enriched in the at least one cannabinoid compound and the one or more additional compounds and depleted in the further compounds, said at least one liquid fraction providing the initial mixture,
- the one or more additional compounds and/or the one or more further compounds comprising pesticides, plant growth regulators or combinations thereof.
A method for the chromatographic purification of at least one cannabinoid compound, wherein the method comprises a main purification stage comprising the steps of:
- injecting an initial mixture comprising the at least one cannabinoid compound and one or more additional compounds onto a main stationary phase comprising silica particles in a chromatographic installation having a non-static bed;
- performing an elution with an elution solution, and collecting one or more elution fractions;
- and
- optionally, washing the main stationary phase with a washing solution and collecting one or more washing fractions;
- wherein
- at least one of the elution fractions or washing fractions contains the at least one cannabinoid compound purified from the one or more additional compounds,
- the elution solution is a mixture of a polar solvent and a non-polar solvent, the polar solvent being an alcohol and the non-polar solvent being an alkane,
- the elution is performed in an isocratic mode, and
- the method further comprises equilibrating the main stationary phase with an equilibration solution in order to equilibrate the main stationary phase for a further injection, the elution solution and the equilibration solution being the same.
A method for the chromatographic purification of at least one cannabinoid compound, wherein the method comprises a main purification stage comprising the steps of:
- injecting an initial mixture comprising the at least one cannabinoid compound and one or more additional compounds onto a main stationary phase comprising silica particles in a chromatographic installation having a non-static bed;
- performing an elution with an elution solution, and collecting one or more elution fractions;
- and then
- washing the main stationary phase with a washing solution and collecting one or more washing fractions;
- wherein
- at least one of the elution fractions or washing fractions contains the at least one cannabinoid compound purified from the one or more additional compounds,
- the elution solution is a mixture of a polar solvent and a non-polar solvent, the polar solvent being an alcohol and the non-polar solvent being an alkane,
- the elution is performed in an isocratic mode, and
- the washing solution is a mixture of a polar solvent and a non-polar solvent, the polar solvent being an alcohol and the non-polar solvent being an alkane.
Dependencies are inferred from the claim text.