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US 12,077,802 B2Granted
Metabolic engineering of E. coli for the biosynthesis of cannabinoid
AssigneeInmed Pharmaceuticals Inc.
InventorsSonal R. Ayakar, Sandip V. Pawar, Steven J. Hallam, Sazzad Hossain, Vikramaditya G. Yadav, Protiva R. Roy, Sarvesh K. Srivastava
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Abstract
Provided herein are methods and compositions for producing terpenoids in a host cell. In some cases, the terpenoids are cannabinoids.
Claims49
An expression cassette comprising a heterologous promoter operably linked to a nucleic acid encoding a bifunctional ispDF enzyme, wherein the bifunctional ispDF enzyme comprises an amino acid sequence at least 85% identical to the amino acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 2, or SEQ ID NO:
- 3, and wherein the the bifunctional ispDF enzyme has 2-C-methyl-D-erythritol 4-phosphate cyticylyltransferase activity and 2-C-methyl-D -erythritol 2,4-cyclodiphosphate synthase activity.
A host cell comprising:
- a. an expression cassette comprising a heterologous promoter operably linked to a nucleic acid encoding a bifunctional ispDF enzyme, wherein the bifunctional ispDF enzyme comprises an amino acid sequence at least 85% identical to the amino acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 2, or SEQ ID NO:
- 3, and wherein the the bifunctional ispDF enzyme has 2-C -methyl-D-erythritol 4-phosphate cyticylyltransferase activity and 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity;
- and
- b. a expression cassette comprising a heterologous promoter operably linked to a nucleic acid encoding a terpenoid synthase.
3 more elements
Dependencies are inferred from the claim text.