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US 12,077,799 B2Granted
Chimeric terpene synthases
AssigneeGinkgo Bioworks, Inc.
InventorsChristian Ridley, Jue Wang, Scott Marr
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Abstract
Described herein are chimeric terpene synthases, methods for making chimeric terpene synthases, and methods for making terpenes using the same.
Claims20
A method for producing one or more terpenes, comprising:
- culturing a fungal cell that comprises a nucleic acid molecule encoding a synthetic terpene synthase, wherein:
- a) the synthetic terpene synthase comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 17, 22, or 29;
- b) the synthetic terpene synthase comprises a DDxx(x)D/E motif;
- and
- c) the fungal cell produces alpha-guaiene.
3 more elements
A method for producing one or more terpenes, comprising:
- culturing a fungal cell that comprises a nucleic acid molecule encoding a synthetic terpene synthase, wherein:
- (a) the synthetic terpene synthase comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 17;
- (b) the synthetic terpene synthase comprises a DDxx(x)D/E motif;
- and
- (c) the fungal cell produces alpha-guaiene.
3 more elements
A method for producing one or more terpenes, comprising:
- culturing a fungal cell that comprises a nucleic acid molecule encoding a synthetic terpene synthase, wherein:
- (a) the synthetic terpene synthase comprises the amino acid sequence of SEQ ID NO:
- 17,22, or 29;
- (b) the synthetic terpene synthase comprises a DDxx(x)D/E motif;
- and
- (c) the fungal cell produces alpha-guaiene.
3 more elements
Dependencies are inferred from the claim text.