Engineered microorganisms for production of 2′fucosyllactose and l-fucose
AssigneesThe Board of Trustees of the University of Illinois, Korea University Research and Business Foundation
InventorsYong-Su Jin, Jingjing Liu, Sora Yu, Eun Ju Yun, Suryang Kwak, Kyoung Heon Kim, Jaewon Lee
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Abstract
Compositions and methods are provided for producing 2′fucosyliaciose and L-fucose from recombinant microorganisms.
Claims12
A recombinant yeast cell comprising heterologous polynucleotides encoding a GDP-mannose 4,6-dehydratase (Gmd) polypeptide, a GDP-4-keto-6-deoxymannose 3,5-epimerase 4-reductase (WcaG) polypeptide, an oligosaccharide transporter polypeptide, a fucosyltransferase polypeptide, a α-L-fucosidase polypeptide, and a L-fucokinase/GDP-L-fucose phosphorylase (FKP) polypeptide, wherein the heterologous polynucleotides are operably linked to at least one expression control polynucleotide.
A vector or combination of vectors comprising:
- a polynucleotide encoding a GDP-mannose 4,6-dehydratase (Gmd) polypeptide wherein the Gmd polypeptide has at least 95% identity to SEQ ID NO:14;
- a polynucleotide encoding GDP-4-keto-6-deoxymannose 3,5-epimerase 4-reductase (WcaG) polypeptide wherein the WcaG polypeptide has at least 95% identity to SEQ ID NO:15;
- a polynucleotide encoding a Lac12 oligosaccharide transporter;
- a polynucleotide encoding fucosyltransferase polypeptide wherein the fucosyltransferase polypeptide has at least 95% identity to SEQ ID NO:13, and a polynucleotide encoding an α-L-fucosidase polypeptide wherein the α-L-fucosidase polypeptide has at least 95% identity to SEQ ID NO:16, wherein the polynucleotides are operably linked to at least one expression control polynucleotide.
Dependencies are inferred from the claim text.