US 12,077,788 B2Granted

Production of a mixture of neutral non-fucosylated oligosaccharides by a cell

AssigneeInbiose N.V.

InventorsSofie Aesaert, Joeri Beauprez, Pieter Coussement, Thomas Decoene, Nausicaä Lannoo, Gert Peters, Kristof Vandewalle, Annelies Vercauteren

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Abstract

The disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, the disclosure is in the technical field of cultivation or fermentation of metabolically engineered cells. The disclosure describes a cell metabolically engineered for production of a mixture of at least four different neutral non-fucosylated oligosaccharides. Furthermore, the disclosure provides a method for the production of a mixture of at least four different neutral non-fucosylated oligosaccharides by a cell as well as the purification of at least one of the oligosaccharides from the cultivation.

Claims27

Dependent claims

A metabolically engineered cell that produces a mixture of at least four different neutral non-fucosylated mammalian milk oligosaccharides, wherein said cell:

is metabolically engineered for production of said mixture,
expresses at least two different glycosyltransferases involved in producing the mixture of at least four different neutral non-fucosylated mammalian milk oligosaccharides, wherein each of said at least two glycosyltransferases is selected from the group consisting of a galactosyltransferase, an N-acetylglucosaminyltransferase, and an N-acetylgalactosaminyltransferase, and
is capable of synthesizing at least one nucleotide-sugar, wherein each nucleotide-sugar is a donor for at least one glycosyltransferase.

Dependencies are inferred from the claim text.