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US 12,077,739 B2Granted
Coating a bioreactor in a cell expansion system
AssigneeTerumo BCT, Inc.
InventorsNathan D. Frank, Brian J. Nankervis, Dennis J. Hlavinka, Thomas G. Dilorenzo
Life of this patent
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Abstract
Embodiments described herein generally provide for the expansion of cells in a cell expansion system using an active promotion of a coating agent(s) to a cell growth surface in some embodiments. A coating agent may be applied to a surface, such as the cell growth surface of a hollow fiber in a bioreactor, by controlling the movement of a fluid in which a coating agent is suspended, by changing flow rates, by changing flow directions, by rotation of the bioreactor, and/or combinations thereof.
Claims9
A method of growing cells in a bioreactor, the method comprising:
- circulating a first fluid at a first flow rate through a bioreactor of a cell expansion system, wherein the first fluid comprises a reagent;
- circulating the first fluid for a first period of time to allow at least a first portion of the reagent to coat the bioreactor;
- after the first period of time, circulating the first fluid at a second flow rate slower than the first flow rate through the bioreactor of the cell expansion system for a second period of time to allow a second portion of the reagent to coat the bioreactor;
- circulating a second fluid through the bioreactor to remove a third portion of the reagent from the bioreactor;
- circulating a third fluid at a third flow rate through a bioreactor of a cell expansion system, wherein the third fluid comprises a plurality of cells;
- reducing circulation of the third fluid from the third flow rate;
- maintaining the bioreactor in a first orientation for a third predetermined period of time to allow at least a portion of the plurality of cells to settle;
- after the third predetermined period of time, rotating the bioreactor to a second orientation that is about 180 degrees from the first orientation;
- after the rotating, maintaining the bioreactor in the second orientation for a fourth period of time to allow at least a second portion of the plurality of cells to settle;
- and
- expanding at least the first and the second portion of cells in the bioreactor.
8 more elements
Dependencies are inferred from the claim text.