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US 12,077,566 B2Granted
Mutants of recombinant immunoregulatory protein of Ganoderma lucidum and applications thereof
AssigneesXitian Zhang, Fei Sun, Zin Zhang
InventorsFei Sun, Chongyang Liang
Life of this patent
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Abstract
Mutants of recombinant immunoregulatory protein of Ganoderma lucidum (rLZ-8) and applications thereof are provided. It is found by the present invention that: an anti-EGFR (epidermal growth factor receptor) domain exists in a structure of the rLZ-8; particularly, the domain through positive potential characteristics thereof induces a killing effect to an abnormal EGFR-expressed tumor. Based on the above scientific discovery, with utilizing computational simulation technology, the mutants of the rLZ-8, having better antitumor effects, are obtained.
Claims9
A Lingzhi-8 (LZ-8;
- SEQ ID NO:
- 1) mutant, wherein the LZ-8 mutant comprises a mutation selected from the group consisting of:
- (1) the amino acid D at position 70 is replaced with K (D70K);
- (2) the amino acid K at position 46 is replaced with E (K46E);
- (3) the amino acid L at position 17 is replaced with K and the amino acid D at position 70 is replaced with K (L17K and D70K);
- (4) the amino acid D at position 20 is replaced with H (D20H);
- (5) the amino acid L at position 17 is replaced with K (L17K);
- (6) the amino acid K at position 46 is replaced with E and the amino acid K at position 74 is replaced with E (K46E and K74E);
- and
- (7) the amino acid D at position 20 is replaced with H and the amino acid D at position 70 is replaced with K (D20H and D70K).
7 more elements
Dependencies are inferred from the claim text.