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US 12,071,660 B2Granted
Bisulfite-free, base-resolution identification of cytosine modifications
AssigneeLudwig Institute for Cancer Research
InventorsChunxiao Song, Yibin Liu
Life of this patent
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Abstract
This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.
Claims19
A method comprising steps of:
- Providing a nucleic acid sample comprising one or more 5-carboxylcytosine (5caC) or 5-formylcytosine (5fC) residues;
- and
- Contacting the sample with a borane reducing agent under conditions that reduce 5caC and 5fC to dihydrouracil (DHU),
- wherein the nucleic acid sample comprising one or more 5caC or 5fC residues comprises ligated nucleic acids.
1 more element
Dependencies are inferred from the claim text.