Pasteurella multocida strains and vaccines having hyaC and nanP deletions
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsRobert E Briggs, Fred M Tatum
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Abstract
The present invention provides novel attenuated Pasteurella multocida strains that may be used, in live or killed form, to formulate vaccines that are highly protective against P. multocida infection in bovines, other mammals, and in birds. The present invention also identifies the combination of nanP and hyaC gene mutations as key to the provision of such vaccines. When appropriately formulated, antigenic material of numerous other bovine pathogens may be combined with the live attenuated Pasteurella multocida strains, to make effective combination vaccines.
Claims10
An attenuated Pasteurella multocida (P. multocida) bacterium wherein the P. multocida bacterium is attenuated by comprising a mutated hyaC gene that encodes a non-functional hyaC polypeptide having the amino acid sequence of SEQ ID NO:11 or encodes a polypeptide having an amino acid sequence at least 80% identical to the amino acid sequence of SEQ ID NO:
- 11 over the length of SEQ ID NO:11 that is in-common with said polypeptide;
- or comprising a mutated nanP gene that encodes a polypeptide unable to take-up sialic acid from growth media having the amino acid sequence of SEQ ID NO:4, or a polypeptide having an amino acid sequence at least 80% identical to the amino acid sequence of SEQ ID NO:4 over the length of SEQ ID NO:4 that is in-common with said polypeptide.
An attenuated P. multocida bacterium comprising a hyaC gene that encodes a non-functional hyaC polypeptide having an amino acid sequence at least 80% identical to the amino acid sequence of SEQ ID NO:11 over the length of SEQ ID NO:11 that is in-common with said polypeptide, and a nanP gene that encodes a polypeptide unable to take-up sialic acid from growth media having an amino acid sequence at least 80% identical to the amino acid sequence of SEQ ID NO:4 over the length of SEQ ID NO:4 that is in-common with said polypeptide.
Dependencies are inferred from the claim text.