RNA target enrichment or depletion of biological samples
AssigneesAltratech Limited, The United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsBrian O'Farrell, Claire O'Connell, Kaylyn Oshaben, Daniel Appella
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Abstract
A method of target enrichment or depletion from a sample with an analyte is described. A probe has one of a left-handed PNA pair and a targeting moiety, in which the left-handed PNA pair are a complementary pair of PNAs that are chiral and have a cyclic backbone modification that induces a left-handed helical structure. A capture surface has the other of the left-handed PNA pair; and the left-handed PNA pair bind to hybridize the probe to the capture surface, which may be a magnetic bead.
Claims16
A method of target enrichment in a sample, the method comprising the steps of:
- introducing a probe comprising a left-handed PNA molecule linked to a capture moiety into a sample comprising target analyte;
- capturing, with the capture moiety, the target analyte;
- and
- binding the left-handed PNA molecule with a complementary PNA molecule comprising a left-handed chiral structure, the complementary PNA molecule being attached to a surface to thereby enrich for target analyte.
Dependencies are inferred from the claim text.