Immunoglobulins directed to bacterial, viral and endogenous polypetides
AssigneeCovalent Bioscience Incorporated
InventorsSudhir Paul, Stephanie Planque, Yasuhiro Nishiyama, Eric L. Brown, Keri C. Smith, Hiroaki Taguchi
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Abstract
Disclosed are antibodies (immunoglobulins) and fragments thereof that hydrolyze or bind polypeptide antigens belonging to Staphylococcus aureus, hepatitis C virus, human immunodeficiency virus and Alzheimer's disease. Also disclosed are novel methods to improve the antigen reactivity of the immunoglobulins and to treat a pathophysiological condition using the immunoglobulins as therapeutics.
Claims17
A method for identifying an immunoglobulin (Ig) variable (V) domain of a light chain subunit (VL) or heavy chain subunit (VH) of an immunoglobulin having a specific, defined antigenic-directed activity comprising:
- performing a screening of a library containing a plurality of IgV domains for the desired antigenic-directed activity, wherein said plurality of IgV domains comprise IgV domains which are a single IgV domain,
- wherein the antigen of said IgV is an amyloid protein or a superantigen other than gp120;
- said IgV comprises a nucleophilic site;
- said IgV catalyzes the hydrolysis of said amyloid protein or superantigen;
- and said IgV is obtained from an organism without prior immunization with said antigen.
A method for identifying an immunoglobulin (Ig) variable (V) domain of a light chain subunit (VL) or heavy chain subunit (VH) of an immunoglobulin having a specific, defined antigenic-directed activity comprising:
- fractionating a library containing a plurality of IgV domains using antigen electrophilic analogs, wherein said plurality of IgV domains comprise IgV domains which are a single IgV domain,
- wherein the antigen of said IgV is an amyloid protein or a superantigen other than gp120;
- said IgV comprises a nucleophilic site;
- said IgV catalyzes the hydrolysis of said amyloid protein or superantigen;
- and said IgV is obtained from an organism without prior immunization with said antigen.
A method for identifying an immunoglobulin (Ig) variable (V) domain of a light chain subunit (VL) or heavy chain subunit (VH) of an immunoglobulin having a specific, defined antigenic-directed activity comprising:
- performing a screening of a library containing a plurality of IgV domains for the desired antigenic-directed activity, wherein said plurality of IgV domains comprise IgV domains which comprise a VL domain linked to a VL domain,
- wherein the antigen of said IgV is an amyloid protein or a superantigen other than gp120;
- said IgV comprises a nucleophilic site;
- said IgV catalyzes the hydrolysis of said amyloid protein or superantigen;
- and said IgV is obtained from an organism without prior immunization with said antigen.
A method for identifying an immunoglobulin (Ig) variable (V) domain of a light chain subunit (VL) or heavy chain subunit (VH) of an immunoglobulin having a specific, defined antigenic-directed activity comprising:
- fractionating a library containing a plurality of IgV domains using antigen electrophilic analogs, wherein said plurality of IgV domains comprise IgV domains which comprise a VL domain linked to a VL domain,
- wherein the antigen of said IgV is an amyloid protein or a superantigen other than gp120;
- said IgV comprises a nucleophilic site;
- said IgV catalyzes the hydrolysis of said amyloid protein or superantigen;
- and said IgV is obtained from an organism without prior immunization with said antigen.
Dependencies are inferred from the claim text.