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US 12,071,340 B2Granted
Integrated analysis devices and related fabrication methods and analysis techniques
AssigneeBionano Genomics, Inc.
InventorsHan Cao, Michael David Austin, Parikshit A. Deshpande, Mark Kunkel, Alexey Y. Sharonov, Michael Kochersperger
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Abstract
Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.
Claims20
A method of fabricating an analysis device, comprising:
- bonding a first substrate and a second substrate,
- the bonding giving rise to an enclosed conduit disposed between the substrates, the enclosed conduit being capable of transporting a fluid therethrough,
- wherein the enclosed conduit comprises a first front-end branched channel region and a nanochannel analysis region,
- wherein the first front-end branched channel region comprises at least a primary channel characterized as having a cross-sectional dimension of from about 10 nm to about 10,000 nm and at least two secondary channels downstream and divided from the primary channel, wherein the total cross-sectional area of the at least two secondary channels is approximately equal to the cross-sectional area of the primary channel, thus maintaining an approximately constant flow rate wherein the ratio of the cross-sectional dimensions of the primary channel to the at least two secondary channels is in the range of 100 to 10,000, wherein the first front-end branched channel region comprises stacked array of channels having progressively reduced cross sectional dimensions, wherein the channels are connected by one to five levels of forks.
1 more element
A method of fabricating an analysis device, comprising:
- bonding a first substrate and a second substrate,
- the bonding giving rise to an enclosed conduit disposed between the substrates, the enclosed conduit being capable of transporting a fluid therethrough,
- wherein the enclosed conduit comprises a first front-end branched channel region and a nanochannel analysis region,
- wherein the first front-end branched channel region comprises at least a primary channel characterized as having a cross-sectional dimension of from about 10 nm to about 10,000 nm and at least two secondary channels downstream and divided from the primary channel, wherein the total cross-sectional area of the at least two secondary channels is approximately equal to the cross-sectional area of the primary channel, thus maintaining an approximately constant flow rate wherein the ratio of the cross-sectional dimensions of the primary channel to the at least two secondary channels is in the range of 100 to 10,000, wherein the first front-end branched channel region comprises five stacked arrays of channels having progressively reduced cross sectional dimensions, wherein the channels are connected by five levels of forks.
1 more element
Dependencies are inferred from the claim text.