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US 12,065,688 B2Granted
Compositions and methods for cellular processing
Assignee10x Genomics, Inc.
InventorJason Bell
Life of this patent
Estimating the term…
Abstract
Provided herein are compositions and methods for cellular analysis. Nucleic acid from single cells may be processed in one or more partitions. A partition may comprise a cell bead and one or more enzymes for nucleic acid processing. A partition may comprise a functionalized polymer. In some cases, single cells may be subjected to epigenetic analysis, thereby generating an epigenetic profile for each cell from a plurality of cells.
Claims19
A method comprising:
- (a) providing a cell bead comprising (i) a nucleus and (ii) a nuclease coupled to a ligand;
- (b) using said nuclease to cleave chromatin in said nucleus, thereby generating a cleaved nucleic acid molecule;
- (c) partitioning (i) said cell bead, (ii) a plurality of nucleic acid barcode molecules, and (iii) a functionalized polymer to generate a partition, wherein said functionalized polymer is selected from the group consisting of dextran, polyethylene glycol (PEG), and polyacrylamide;
- (d) outside of said cell bead in said partition, using said functionalized polymer to bind to said nuclease via said ligand;
- and
- (e) using said cleaved nucleic acid molecule or a derivative thereof and a nucleic acid barcode molecule of said plurality of nucleic acid barcode molecules to perform one or more reactions in said partition to generate a barcoded nucleic acid molecule.
3 more elements
The method of 1, wherein said nuclease is an engineered nuclease.
Dependencies are inferred from the claim text.