Selection method of iPS cell, preparation method of iPS cell, and control device
AssigneeOlympus Corporation
InventorRyutaro Akiyoshi
Life of this patent
Estimating the term…
Abstract
A selection method of an iPS cell includes: at a reprogramming process to culture a cell including a plurality of combinations of initializing factors labelled with luminescent genes that are different with each other, acquiring a photon number per unit area or a photon number per unit time of each of the luminescent genes of the cell; judging whether the acquired photon number is more than a threshold that is predetermined for the acquired photon number; and when the acquired photon number is more than the threshold, selecting this cell as an objective cell for a next process.
Claims17
A selection method of an iPS cell, the selection method comprising:
- at a reprogramming process to culture a cell including a plurality of combinations of initializing factors labelled with luminescent genes that are different with each other, acquiring a photon number per unit area or a photon number per unit time of each of the luminescent genes of the cell;
- judging whether the acquired photon number is more than a threshold that is predetermined for the acquired photon number;
- and
- when the acquired photon number is more than the threshold, selecting this cell as an objective cell for a next process.
A preparation method of an iPS cell, the preparation method comprising:
- at a reprogramming process to culture a cell including a plurality of combinations of initializing factors labelled with luminescent genes that are different with each other, acquiring a luminescence amount of each of the luminescent genes of the cell;
- judging whether the acquired luminescence amount is more than a threshold that is predetermined for the acquired luminescence amount;
- when the acquired luminescence amount is more than the threshold, selecting this cell as an objective cell for a next process;
- and
- transplanting the selected cell to a new culture vessel, repeatedly up to a prescribed number of times.
A control device comprising a processor comprising hardware, the processor being configured to:
- acquire, at a reprogramming process to culture a cell including a plurality of combinations of initializing factors labelled with luminescent genes that are different with each other, a photon number per unit area or a photon number per unit time of each of the luminescent genes of the cell;
- judge whether the acquired photon number is more than a threshold that is predetermined for the acquired photon number;
- and
- when the acquired photon number is more than the threshold, select this cell as an objective cell for a next process.
Dependencies are inferred from the claim text.