Promoter for heterologous expression
AssigneeBASF SE
InventorsMax Fabian Felle, Bogdan Tokovenko
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Abstract
The present invention is directed to a nucleic acid construct comprising a polynucleotide operably linked to one or more control sequence that directs the expression of the polynucleotide in a host cell, wherein at least one control sequence comprises a promoter sequence of an operon comprising a secA gene or a functional fragment or functional variant thereof and wherein said promoter sequence is heterologous to the polynucleotide. In a further embodiment, the invention is directed to an expression vector and a host cell comprising the nucleic acid construct comprising the promoter sequence described herein and a method of expressing a polynucleotide in a host cell.
Claims9
A recombinant nucleic acid construct comprising a polynucleotide operably linked to one or more control sequence that directs the expression of the polynucleotide in a host cell, wherein at least one control sequence comprises a Bacillus secA promoter sequence and wherein said promoter sequence is heterologous to the polynucleotide, wherein the polynucleotide does not comprise a reporter gene and the polynucleotide does not comprise a secA gene, and wherein the polynucleotide encodes for a protein of interest, wherein the protein of interest is a methyltransferase.
Dependencies are inferred from the claim text.