Expression of functional bacterial type I fatty acid synthase in photosynthetic hosts
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsWillem Vermaas, Tyson Burch
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Abstract
Provided herein are compositions and methods for expression of functional bacterial type I fatty acid synthase in photosynthetic hosts for production of activated acyl-CoAs and for use in biofuel production.
Claims4
A genetically modified photosynthetic Synechocystis sp. PCC 6803 bacterium which produces activated acyl-CoA, the Synechocystis sp. PCC 6803 bacterium comprising one or more transgenes expressing a heterologous bacterial fatty acid synthase type I (FAS I) from Rhodococcus fascians (R. fascians) D188 and a heterologous acyl carrier protein synthase (acpS) from Rhodococcus fascians (R. fascians) D188 driven by a nickel-inducible promoter, wherein the genetically modified photosynthetic Synechocystis sp. PCC 6803 bacterium is capable of increased production of activated acyl-CoA compared to a genetically unmodified photosynthetic Synechocystis sp. PCC 6803 bacterium, wherein the genetically unmodified photosynthetic Synechocystis sp. PCC 6803 bacterium does not express endogenous FAS I.
A genetically modified Synechocystis sp. PCC 6803 strain expressing a transgene encoding a heterologous bacterial fatty acid synthase type I (FAS I) from Rhodococcus fascians (R. fascians) D188 driven by a nickel-inducible promoter heterologous to the FAS I coding sequence and capable of driving expression within the Synechocystis sp. PCC 6803 strain;
- wherein the modified Synechocystis sp. PCC 6803 strain is capable of increased production of activated acyl-CoA compared to an unmodified Synechocystis sp. PCC 6803 strain lacking the heterologous bacterial FAS I.
Dependencies are inferred from the claim text.