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US 12,064,735 B2Granted
Oscillating bioreactor system
AssigneeLifecycle Biotechnologies, LP
InventorsCody Yarborough, Ronald Gohdes
Life of this patent
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Abstract
A biological production system includes a mixing vessel and an agitation device. The mixing vessel includes an outer housing, an internal mixing structure, and a reaction chamber. The mixing vessel is configured to provide low shear agitation to materials in the reaction chamber in response to force from the agitation device.
Claims24
A mixing vessel, comprising:
- a rigid outer housing formed of a single unitary material and comprising a closed bottom end, a top end opposite the bottom end including a neck defining an opening configured to be sealed with a closure, and a cylindrical wall integrally formed with, and extending between, the bottom end and the top end, the rigid outer housing tapering from the cylindrical wall to the neck such that a diameter of the cylindrical wall is greater than a diameter of the neck;
- a reaction chamber within the housing;
- and
- an internal mixing structure comprising at least two raised surfaces integrally formed with, and extending from, the cylindrical wall into the reaction chamber;
- wherein each of the at least two raised surfaces extends from 90 degrees to 270 degrees around a circumference of the cylindrical wall;
- wherein the internal mixing structure completely traverses the circumference of the cylindrical wall between the bottom end and the top end;
- and
- wherein the mixing vessel is configured to provide low shear agitation to materials contained within the reaction chamber in response to an externally applied force.
5 more elements
A biological production system, comprising:
- a mixing vessel comprising:
- a rigid outer housing comprising a closed bottom end, a top end opposite the bottom end including a neck defining an opening, and a cylindrical wall integrally formed with, and extending between, the bottom end and the top end, the rigid outer housing tapering from the cylindrical wall to the neck such that a diameter of the cylindrical wall is greater than a diameter of the neck;
- a reaction chamber within the housing;
- and
- an internal mixing structure comprising at least two raised surfaces integrally formed with, and extending from, the cylindrical wall into the reaction chamber;
- wherein each of the at least two raised surfaces extend from 90 degrees to 270 degrees around a circumference of the cylindrical wall;
- wherein the internal mixing structure completely traverses the circumference of the cylindrical wall between the bottom end and the top end;
- and
- wherein the outer housing and the internal mixing structure are formed of a single unitary material;
- a closure configured to seal the opening;
- and
- an agitation device;
- wherein the mixing vessel is configured to provide low shear agitation to materials contained within the reaction chamber in response to an externally applied force from the agitation device.
10 more elements
A method of mixing cells, the method comprising:
- providing a mixing vessel comprising:
- a rigid outer housing comprising a closed bottom end, a top end opposite the bottom end including a neck defining an opening, and a cylindrical wall integrally formed with, and extending between, the bottom end and the top end, the rigid outer housing tapering from the cylindrical wall to the neck such that a diameter of the cylindrical wall is greater than a diameter of the neck;
- a reaction chamber within the housing;
- and
- an internal mixing structure comprising at least two raised surfaces integrally formed with, and extending from, the cylindrical wall into the reaction chamber;
- wherein each of the at least two raised surfaces extend from 90 degrees to 270 degrees around a circumference of the cylindrical wall;
- wherein the internal mixing structure completely traverses the circumference of the cylindrical wall between the bottom end and the top end;
- and
- wherein the outer housing and the internal mixing structure are formed of a single unitary material;
- depositing cells in a liquid medium into the reaction chamber;
- and
- agitating the mixing vessel using an agitation device.
9 more elements
Dependencies are inferred from the claim text.