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US 12,060,608 B2Granted
Sequencing and high resolution imaging
AssigneePacific Biosciences of California, Inc.
InventorsBryan P. Staker, Niandong Liu, Manohar R. Furtado, Rixun Fang, Norman Burns, Windsor Owens
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Abstract
Disclosed herein are methods and systems for detection and discrimination of optical signals from a densely packed substrate. There have broad applications for biomolecule detection near or below the diffraction limit of optical systems, including in improving the efficiency and accuracy or polynucleotide sequencing applications.
Claims20
A method for processing or analyzing a plurality of analytes, comprising:
- (a) providing said plurality of analytes on a substrate;
- (b) obtaining a plurality of optical signals from a plurality of probes over one or more cycles of said plurality of probes binding to one or more analytes of said plurality of analytes;
- (c) determining a first peak intensity of a first analyte of said plurality of analytes at a first peak location and determining a second peak intensity of a second analyte of said plurality of analytes at a second peak location, wherein said first peak location and said second peak location are determined with no more than 10 nanometer root mean square error, and wherein said first peak intensity and said second peak intensity at least partially overlap;
- and
- (d) processing said first peak intensity and said second peak intensity to distinguish said first analyte from said second analyte.
2 more elements
A system for processing or analyzing a plurality of analytes, comprising:
- (a) a substrate configured to support said plurality of analytes immobilized adjacent thereto;
- (b) an optical imaging module configured to obtain a plurality of optical signals from said substrate over a plurality of cycles of a plurality of probes binding to one or more analytes of said plurality of analytes immobilized adjacent to said substrate;
- and
- (c) an image processing module configured to:
- (i) determine a first peak intensity of a first analyte of said plurality of analytes at a first peak location and a second peak intensity of a second analyte of said plurality of analytes at a second peak location, wherein said first peak location and said second peak location are determined with no more than 10 nanometer root mean square error, and wherein said first peak intensity and said second peak intensity at least partially overlap;
- and
- (ii) process said first peak intensity and said second peak intensity to distinguish said first analyte from said second analyte.
4 more elements
Dependencies are inferred from the claim text.