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US 12,060,593 B2Granted
Fucosyltransferases and their use in producing fucosylated oligosaccharides
AssigneeChr. Hansen HMO GmbH
InventorsStefan Jennewein, Katja Parschat
Life of this patent
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Abstract
Fucosyltransferases capable of transferring a fucose residue from a donor substrate to a lactotetraose, methods for producing fucosylated oligosaccharides utilizing the fucosyltransferases, and the use of the thus produced fucosylated oligosaccharides for manufacturing nutritional compositions are provided.
Claims12
A method for producing a fucosylated oligosaccharide, the method comprising:
- a) providing at least one genetically engineered cell that has been engineered to express a heterologous fucosyltransferase, wherein the heterologous fucosyltransferase comprises an amino acid sequence having at least 80% sequence identity to one of SEQ ID NOs:
- 17-26 and 28-30, wherein the fucosyltransferase is capable of transferring a fucose residue from a donor substrate to an acceptor molecule;
- b) cultivating by fermentation the at least one genetically engineered cell in the presence of at least one carbon source, an acceptor molecule being a lactotetraose, and a donor substrate, comprising a fucose residue, and under conditions suitable for the at least one genetically engineered cell to transfer the fucose residue from the donor substrate to the acceptor molecule to produce the fucosylated oligosaccharide.
Dependencies are inferred from the claim text.