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US 12,060,574 B2Granted
Methods of generating tissue using devitalized, acellular scaffold matrices derived from micro-organs
AssigneeYissum Research Development Company of the Hebrew University of Jerusalem
InventorEduardo N. Mitrani
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Abstract
A composition of matter is provided comprising a devitalized, acellular tissue-derived scaffold seeded with differentiated cells, particularly pancreatic islet cells, wherein the cells can maintain cell-specific function or structure in culture on the scaffold. Methods of generating same and uses thereof are also provided.
Claims12
A composition of matter comprising:
- (i) a devitalized, acellular, lung tissue-derived three dimensional scaffold having dimensions selected such that the point deepest within said scaffold is at least about 100 micrometers and not more than about 225 micrometers away from the nearest surface of the scaffold;
- and (ii) beta cells selected from beta cells isolated from pancreatic islets and beta cells differentiated in-vitro from stem and/or progenitor cells,
- wherein the beta cells are seeded and cultured on said acellular three dimensional scaffold and maintain glucose-responsive insulin secretion for at least 7 days of culture on said scaffold.
Dependencies are inferred from the claim text.