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US 12,060,570 B2Granted
Vector-free intracellular delivery by reversible permeabilisation
AssigneeAvectas Limited
InventorsMichael Maguire, Shirley O'Dea
Life of this patent
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Abstract
The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.
Claims51
A system comprising:
- a plate comprising a well, wherein the plate comprises a population of cells,
- a housing configured to receive the plate comprising a well;
- a differential pressure applicator configured to apply a differential pressure to the well;
- a delivery solution applicator configured to deliver atomized delivery solution to the well;
- a reservoir fluidically coupled to the delivery solution applicator, the reservoir including a delivery solution including an aqueous solution including a payload,
- wherein the aqueous solution is isotonic,
- wherein the population of cells is contacted with a volume of aqueous solution by gas propelling the aqueous solution to form a spray,
- wherein the payload comprises a gene editing composition;
- a stop solution applicator configured to deliver a stop solution to the well;
- and
- a culture medium applicator configured to deliver a culture medium to the well.
8 more elements
An in vitro method comprising:
- providing a population of cells within a well of a system including a housing configured to receive a plate comprising the well,
- a differential pressure applicator configured to apply a differential pressure to the well,
- a delivery solution applicator configured to deliver atomized delivery solution to the well,
- a reservoir fluidically coupled to the delivery solution applicator, the reservoir including a delivery solution, the delivery solution including an aqueous solution including a payload and an alcohol at greater than 5 percent (v/v) concentration, wherein the aqueous solution is isotonic, wherein the payload comprises a gene editing composition,
- and a culture medium applicator configured to deliver a culture medium to the well, and
- contacting the population of cells with a volume of aqueous solution by gas propelling the aqueous solution to form a spray.
3 more elements
Dependencies are inferred from the claim text.