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US 12,054,862 B2Granted
Electrospun PNIPAAm/PCL fiber mats for aligned cell sheets
AssigneeBoard of Regents, The University of Texas System
InventorsJaneta Zoldan, Alicia Allen
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Abstract
The present invention provides compositions comprising aligned fibers of electrospun PNIPAAm and poly (ε-caprolactone) (PCL) (denoted PNIPAAm/PCL fibers). The PNIPAAm/PCL compositions enable enhanced growth and detachment of intact anisotropic cell sheets. The compositions do not require chemical modification or resource-intensive techniques, thus saving time and expense, and have the potential to generate tissue-specific, aligned cell sheets for transplant studies.
Claims12
A method of making an anisotropic cell sheet, comprising the steps of:
- electrospinning a solution comprising poly(N-isopropylacrylamide (PNIPAAm) and poly(caprolactone) (PCL) having a PNIPAAm to PCL ratio of at least 90% to generate a fiber mat having fibers in substantially parallel alignment;
- culturing cells on the fiber mat in an environment above about 32° C. to form an anisotropic sheet of cells attached to the fiber mat;
- and
- introducing the fiber mat to an aqueous environment below about 32° C. to release an intact anisotropic sheet of cells.
1 more element
Dependencies are inferred from the claim text.