Meta-stable oligonucleotides junctions for delivery of therapeutics
AssigneesCity of Hope, California Institute of Technology
InventorsSi-ping Han, Marwa Ben Haj Salah, Lisa Scherer, William A. Goddard, John J. Rossi
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Abstract
Disclosed herein are multi-way oligonucleotide junctions for delivering one or more cargo molecules to a biological target and method of making such junctions. The oligonucleotide junctions are formed by two or more oligonucleotides and are stable outside the cell and easily dissociate inside the cell to release the cargo molecule(s). One or more cargo molecules as well as delivery ligand can be loaded to the junctions for targeted delivery. Also disclosed are nanostructures including one or more junctions attached to each other for delivering two or more cargo molecules.
Claims19
A multi-way oligonucleotide junction for delivering one or more cargo molecules, comprising three or more oligonucleotides, wherein at least a portion of each oligonucleotide complementarily binds to a portion of another oligonucleotide to form at least three double-stranded arms, wherein at least one base pair of each of the at least three double-stranded arms is crosslinked such that the junction is stable during storage or circulation and readily dissociates upon delivery to a target, and wherein at least one of the three or more oligonucleotides comprises or is attached to at least one single-stranded overhang.
Dependencies are inferred from the claim text.