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US 12,053,775 B2Granted
Compositions, devices, and methods of mitigating lipoprotein interference in in vitro diagnostic assays for hydrophobic analytes
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsTie Wei, Jie Li, Yun Yue
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Abstract
Methods of mitigating lipoprotein interference in in vitro diagnostic assays for target hydrophobic analytes are disclosed, as well as compositions, kits, and devices useful in said methods. A pretreatment reagent is utilized that includes at least one enzyme that digests lipoprotein.
Claims12
A method for detecting the presence and/or concentration of a target hydrophobic analyte in a biological sample, the method comprising the steps of:
- combining, either simultaneously or wholly or partially sequentially:
- (1) the biological sample suspected of containing the target hydrophobic analyte;
- (2) a pretreatment reagent that digests lipoprotein particles, wherein the pretreatment reagent comprises protease, lipase, and a cofactor for lipase;
- and
- (3) at least one assay reagent capable of detecting the target hydrophobic analyte;
- and
- performing a detection assay using (3) to determine a concentration of target hydrophobic analyte present in the biological sample.
4 more elements
Dependencies are inferred from the claim text.