Method of detecting antibodies against JEV or ZIKV using recombinant baculoviruses expressing prM/e
AssigneeChung Yuan Christian University
InventorsTzong-Yuan Wu, Szu-Cheng Kuo, Pei-Yun Shu, Chang-Chi Lin, Der-Jiang Chiao, Ying-Ju Chen, Yi-Ting Lin, Shu-Fen Chang, Chien-Ling Su
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Abstract
Disclosed herein are recombinant baculoviruses suitable for detecting the presence of arthropod-borne viruses in a biological sample of a test subject. The information derived from the detection may also be used to render a diagnosis on whether the test subject is infected with the arthropod-borne viruses or not, so that proper course of treatment may be assigned to the subject.
Claims3
A method of detecting an antibody against Japanese encephalitis virus (JEV) or Zika virus (ZIKV) in a biological sample comprising:
- mixing the biological sample containing the antibody against the JEV or ZIKV with a recombinant baculovirus having pre-M and E of the JEV or ZIKV expressed thereon;
- and
- detecting a complex formed between the antibody against the JEV or ZIKV and the recombinant baculovirus having the pre-M and E of the JEV or ZIKV expressed thereon in an enzyme-linked immunosorbent assay (ELISA).
Dependencies are inferred from the claim text.