Vacuum liquid extraction and purification systems and methods

A vacuum system for purification of and extraction of at least one substance from a liquid sample comprising:

• a rotatable base for rotation between at least a first position and a second position;
• at least one assay column assembly having at least a first assay column and at least one second assay column;
• at least one Stage 1 solvent reservoir and having at least one easy connect fitting configured for fluidic and reversible connection to the first assay column;
• at least one Stage 1 manifold mounted on a Stage 1 portion of the rotatable base and having at least one easy connect fitting configured for fluidic and reversible connection with the second assay column;
• at least one Stage 2 reservoir and having at least one easy connect fitting configured for fluidic and reversible connection with the second assay column;
• at least one Stage 2 manifold mounted on a Stage 2 portion of the rotatable base and having at least one easy connect fitting configured for fluidic and reversible connection with the second assay column;
• wherein the first assay column has an easy connect fitting disposed at a first entry which is configured for fluidic and reversible connection to the easy connect fitting of the Stage 1 solvent reservoir;
• wherein the first assay column has an easy connect fitting disposed at a first exit opposing the first entry of the first assay column which is configured for fluidic and reversible connection to the second assay column;
• wherein the second assay column has an easy connect fitting disposed at a first entry of the second assay column which is configured for fluidic and reversible selective alternate connection with the first assay column and the Stage 2 manifold;
• wherein the second assay column has an easy connect fitting disposed at a first exit opposing the first entry of the first assay column which configured for fluidic and reversible selective alternate connection with the Stage 1 manifold and the Stage 2 solvent reservoir, and optionally with a third assay column;
• a vacuum pump configured for selective fluidic coupling with the Stage1 manifold and the Stage 2 manifold;
• the vacuum system comprising:
• at least one sample container for containing a liquid sample;
• a drying gas manifold;
• and
• an easy connect fitting disposed at an opposing second end of at least one of the first, second and third assay columns for fluidic and reversible selective alternate connection to the corresponding at least one fluid sample fluid container, the drying gas manifold and a Stage 2 solvent reservoir.