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AssigneeJiangnan University
InventorsLiming Liu, Guipeng Hu, Xiulai Chen, Danlei Ma, Jia Liu, Qiuling Luo
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The disclosure discloses construction and application of an engineered strain of E. coli for producing malic acid by fixing CO2, and belongs to the field of fermentation. The engineered strain is obtained by performing genetic engineering transformation on Escherichia coli MG1655; the genetic engineering transformation includes knocking out a fumarate reductase gene, a fumarase gene, a lactate dehydrogenase gene and an alcohol dehydrogenase gene and freely overexpressing a formate dehydrogenase, an acetyl coenzyme A synthetase, an acylated acetaldehyde dehydrogenase, a formaldehyde lyase, a dihydroxyacetone kinase, a malic enzyme and a phosphite oxidoreductase to obtain a strain GH0407. The strain is used for producing malic acid by fermentation, anaerobic fermentation is performed for 72 hours with CO2 and glucose as a co-substrate, the production of malic acid reaches 39 g/L, the yield is 1.53 mol/mol, and accumulation of malic acid in the original strain is not achieved.
An engineered strain of E. coli for producing malic acid by fixing CO2, wherein a fumarate reductase gene (frdBC), a fumarase gene (fumB), a lactate dehydrogenase gene (IdhA) and an alcohol dehydrogenase gene (adhE) of the engineered strain of E. coli are knocked out, and a formate dehydrogenase (FDH), an acetyl coenzyme A synthetase (ACS), an acylated acetaldehyde dehydrogenase (ACDH), a formaldehyde lyase (FLS), a dihydroxyacetone kinase (DHAK), a malic enzyme (ME) and a phosphite oxidoreductase (PTXD) are overexpressed;
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