Unglycosylated lysostaphin variant protein
AssigneeTrustees of Dartmouth College
InventorsKarl E. Griswold, Hongliang Zhao
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Abstract
Unglycosylated lysostaphin variant protein, nucleic acid molecule, vector and host cell, as well as a method for production of unglycosylated lysostaphin variant protein in a yeast expression system are provided. The proteins are produced in a Pichia pastoris expression system and have been shown to have activity equivalent to wild-type lysostaphin. The lysostaphin variant proteins can be used as therapeutic proteins for treatment of diseases such as Staphylococcus aureus infection.
Claims17
A vector comprising a recombinant nucleic acid molecule encoding a lysostaphin protein or variant thereof, wherein the recombinant nucleic acid molecule encoding the lysostaphin protein or variant thereof comprises the nucleotide sequence of SEQ ID NO:3, SEQ ID NO:5, or SEQ ID NO:7, and wherein the lysostaphin variant protein has endopeptidase activity.
Dependencies are inferred from the claim text.