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US 12,049,646 B2Granted
SC-beta cells and compositions and methods for generating the same
AssigneePresident and Fellows of Harvard College
InventorsQuinn P. Peterson, Felicia J. Pagliuca, Douglas A. Melton, Jeffrey Robert Millman, Michael Saris Segel, Mads Gurtler
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Abstract
Disclosed herein are methods, compositions, kits, and agents useful for inducing β cell maturation, and isolated populations of SC-β cells for use in various applications, such as cell therapy.
Claims30
A method of administering a composition to a subject with Type 1 Diabetes, comprising the step of administering to the subject a composition comprising one or more cell clusters;
- wherein the one or more cell clusters comprise a plurality of cells that express glucagon and/or somatostatin;
- and wherein at least 20% of the cells in the one or more cell clusters are non-native pancreatic β-cells that:
- (a) express chromogranin A, ISL1, PDX1, and C-peptide and one or more of the following genes:
- ZNT8, MNX1, or ABCC8;
- (b) exhibit an in vitro glucose-stimulated insulin secretion response when subjected to a glucose challenge;
- and
- (c) do not express somatostatin or glucagon.
4 more elements
Dependencies are inferred from the claim text.