Compact reverse flow centrifuge system

A compact reverse flow centrifuge system comprising:

• a reusable subsystem;
• and
• a single use, replaceable subsystem, the reusable subsystem comprising:
• a rotating motor head;
• a peristaltic pump;
• a valve assembly comprising one or more selectively operable pinch valve actuators;
• a system controller configured to control operation of the rotating motor head, the peristaltic pump, and the valve assembly in accordance with a programmed processing protocol;
• and
• case housing the rotating motor head, the peristaltic pump, and the valve assembly, and the single use, replaceable subsystem comprising:
• a separation chamber comprising a substantially conical fluid enclosure portion connected to a neck portion, and having a dip tube extending centrally through the conical fluid enclosure from the neck portion to a conical tip of the conical fluid enclosure to provide a dip tube fluid path to the conical tip of the conical fluid enclosure, the neck portion further comprising an elution fluid path;
• a fluid delivery manifold comprising a first fluid port and a second fluid port configured for fluid communication with the separation chamber, a plurality of manifold fluid paths configured for connection to external fluid supply components for delivery of fluid to or from the first fluid port and the second fluid port, at least one of the plurality of manifold fluid paths being configured for engagement with at least one of the one or more pinch valve actuators of the valve assembly whereby the plurality of manifold fluid paths can be selectively opened or closed by operation of the one or more selectively operable pinch valve actuators of the valve assembly, and a pump engagement portion configured to enable operable engagement between the peristaltic pump and fluid paths to cause fluid flow within the plurality of manifold fluid paths of the fluid delivery manifold by operation of the peristaltic pump;
• and
• a rotary coupling connecting to the neck portion of the separation chamber through a rotational axis of the rotary coupling to connect the fluid delivery manifold to the separation chamber, and the rotary coupling having formed therein a first fluid communication path between the dip tube and the first fluid port, and a second fluid communication path between the elution fluid path and the second fluid port, the rotary coupling being configured to enable rotation of the separation chamber about the rotational axis through the neck portion relative to the fluid delivery manifold while the fluid delivery manifold is held in a fixed position by the case, and wherein the first fluid communication path communicating with the dip tube is aligned with the rotational axis,
• the neck portion of the separation chamber being further configured to engage with the rotating motor head which, in use, causes rotation of the separation chamber about the rotational axis, and
• the single use, replaceable subsystem being further configured to provide a closed environment for execution of reverse flow centrifugation processes.