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US 12,044,683 B2Granted
Methods and compositions concerning intestinal permeability
AssigneeUniversity of Florida Research Foundation, Inc.
InventorAli Zarrinpar
Life of this patent
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Abstract
Methods, assays and compositions for measuring intestinal permeability in subjects are provided. The methods and assays comprise the measurement of the level of food dyes, such as FD&C Blue No. 1, in blood samples shortly after the ingestion of the compositions. The methods disclosed herein are simple, cost-effective, reproducible and sensitive.
Claims14
A method for evaluating the integrity of an intestinal barrier in a mammalian subject comprising:
- a) administering to the mammalian subject a pharmaceutical composition comprising from 0.1 mg/kg to 1 mg/kg of disodium;
- 2-[[4-[ethyl-[(3-sulfonatophenyl)methyl]amino]phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]methyl]benzenesulfonate (“Blue #1”);
- b) assaying or measuring Blue #1 in a blood sample collected from the mammalian subject within 24 hours after the mammalian subject has been administered the pharmaceutical composition;
- and
- c) evaluating the integrity of the intestinal barrier of the mammalian subject based on the amount of Blue #1 measured in the blood sample;
- wherein the pharmaceutical composition is administered to the mammalian subject orally or nasogastrically;
- wherein the pharmaceutical composition further comprises a positive control dye;
- and
- wherein the positive control dye is 3,3′-dioxo-2,2′-bisindolyden-5,5′-disulfonic acid disodium salt.
6 more elements
Dependencies are inferred from the claim text.