Advanced production of cannabinoids in yeast

A method for making Cannabigerovarinic Acid in yeast or fungi, the method comprising:

• converting glucose to malonyl-CoA with a first nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 124 expressing Meningitis associated and temperature regulated Fimbria (MatB);
• converting the malonyl-CoA to Acetoacetyl-CoA with a second nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 118 expressing Monocarboxylate Transporter 1 (MCT1);
• converting the Acetoacetyl-CoA to 3-Hydroxybutyryl-CoA with a third nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 119 expressing 3-hydroxybutyryl-CoA dehydrogenase (Hbd);
• converting the 3-Hydroxybutyryl-CoA to Crotonyl-CoA with a fourth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 120 expressing crotonase (Crt);
• converting the Crotonyl-CoA to Butyryl-CoA with a fifth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 123 expressing trans-enoyl-CoA reducatase (Ter);
• converting the Butyryl-CoA to divarinic acid with a sixth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 60 expressing Polyketide synthase and with a seventh nucleotide sequence of SEQ. ID. NO. 92 or SEQ. ID. NO. 93 expressing olivetolic acid cyclase;
• and
• converting the divarinic acid to Cannabigerovarinic Acid with an eighth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 30 expressing an Aromatic prenyltransferase,
• wherein the yeast or fungi is selected from the group consisting of S. cerevisiae, K. marxianus, Y. lipolytica, Aspergillus oryzae, Aspergillus nidulans, and Komagataella pastoris.