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US 12,043,849 B2Granted
Protein purification method and kit
AssigneeExothera L.L.C.
InventorsJosé Castillo, Vasily Medvedev
Life of this patent
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Abstract
The invention provides a method and a kit for combined virus inactivation and capture/purification of a feed containing a protein of interest. The method comprises the steps of: contacting a feed comprising a protein of interest with negatively charged particles or media, conditioning the particles or media such that the pH is acidic thereby inactivating said virus, and eluting said protein of interest.
Claims13
A method for combined virus inactivation and purification of a feed containing a protein of interest comprising the steps of:
- (a) contacting the feed with negatively charged particles or media,
- (b) conditioning the particles or media such that their pH is at least 2.5 and at most 4.3 for at least one minute after the particles or media contact the feed to inactivate one or more viruses present in said feed, and
- (c) eluting said protein of interest from said particles or media.
Dependencies are inferred from the claim text.