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US 12,042,533 B2Granted
Irradiation-inactivated poliovirus, compositions including the same, and methods of preparation
AssigneesBiological Mimetics, Inc., The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.
InventorsGregory J. Tobin, Michael J. Daly
Life of this patent
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Abstract
Compositions useful as poliovirus immunogens are provided along with methods and compositions for preparing the same. Compositions comprising poliovirus immunogens can enable a host response that includes virus-neutralizing antibodies which can protect the host from infection and/or disease.
Claims17
A method of producing an inactivated poliovirus immunogen, the method comprising:
- exposing a poliovirus to a divalent cation which is Mn2+, a peptide which comprises any one or any combination of SEQ ID NO:1-3, and a phosphate buffer;
- and then
- exposing the poliovirus in the presence of the divalent cation, peptide, and buffer to ultraviolet radiation in an amount sufficient to inactivate the poliovirus, thereby providing the inactivated poliovirus immunogen, wherein the poliovirus immunogen stimulates neutralizing antibodies as determined in the standard Wistar rat model when administered to a subject.
A method of producing an inactivated poliovirus immunogen, the method comprising:
- exposing a poliovirus to a divalent cation which is Mn2+, a peptide which comprises any one or any combination of SEQ ID NO:1-3, and a phosphate buffer;
- at least partially replacing air in contact with the poliovirus and/or in a container comprising the poliovirus with a non-reactive gas;
- and then
- exposing the poliovirus to ionizing radiation and/or ultraviolet radiation in an amount sufficient to inactivate the poliovirus, thereby providing the inactivated poliovirus immunogen.
1 more element
Dependencies are inferred from the claim text.