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US 12,038,433 B2Granted
Methods and apparatus for high-throughput screening for testing permeability and retention of compounds across biological barriers
AssigneeBoard of Regents, The University of Texas System
InventorsHugh Smyth, Patricia Pereira Martins
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Abstract
Devices, systems and methods to directly quantify molecular entities and, compounds, inactive ingredients, compositions or formulations across and into specific layers of biological membranes, such as the skin. Exemplary methods enable the determination of the amount of drug into and across tissues as well as the measurement of the effect of chemicals/compounds on membranes, mainly the skin.
Claims9
A method of analyzing membrane retention and permeation, the method comprising:
- securing a biological barrier between a first plurality of wells and a second plurality of wells;
- contacting the biological barrier with a compound, wherein:
- the compound is initially contained in the first plurality of wells;
- the compound is in contact with the biological barrier for a period of time;
- and
- the compound comprises an optically detectable compound and a therapeutic agent;
- removing the biological barrier from between the first plurality of wells and the second plurality of wells;
- analyzing the biological barrier to determine an amount of the optically detectable compound retained in the biological barrier, wherein:
- the biological barrier comprises a dermal or epidermal membrane;
- analyzing the biological barrier to determine the amount of the optically detectable compound retained in the biological barrier comprises an optical analysis of the biological barrier;
- and
- the optical analysis comprises:
- directing an excitation light toward the biological barrier;
- and
- measuring an emitted light from the biological barrier.
12 more elements
Dependencies are inferred from the claim text.