Purifying a sample solution via real-time multi-angle light scattering

A system comprising:

• a memory;
• and
• a processor in communication with the memory, the processor configured to perform a method comprising:
• receiving from a multi-angle light scattering (MALS) instrument baseline scattering intensity values collected over a first period of time of a pure buffer flowed from a chromatography system to the MALS instrument,
• in response to receiving the baseline scattering intensity values of the pure buffer, receiving from the MALS instrument scattering intensity values collected over a second period of time of a sample solution flowed from the chromatography system to the MALS instrument, the second period of time being after the first period of time;
• in response to receiving the baseline scattering intensity values of the pure buffer following a measurement to determine the baseline scattering intensity values during the first period of time and in response to receiving the scattering intensity values of the sample solution, characterizing at least one component of the sample solution, resulting in a time set of values of a dimension, D, of the at least one component and a real-time set of values of excess Rayleigh ratio, R0, of the at least one component,
• wherein the values of the dimension, D, correspond to one of a root-mean-square (rms) radius, Rg, of the at least one component, a sphere radius, R, of the at least one component, a rod length, L, of the at least one component, and a shape dimension relevant to a shape of the at least one component,
• determining that the values of the dimension, D, fall within a dimension, D, value range and that the values of excess Rayleigh ratio, R0, fall within an excess Rayleigh ratio, R0, value range,
• wherein the values of the excess Rayleigh ratio, R0, within the excess Rayleigh ratio, R0 value range indicate that a sufficient signal has been received from the MALS instrument for a reliable calculation of the values of the dimension, D
• in response to determining that the values of the dimension, D, fall within the dimension, D, value range, transmitting a collect sample solution command to collect the sample solution in a product pool,
• determining that at least one of the values of the dimension, D, do not fall within the dimension, D, value range, and
• in response to determining that at least one of the values of the dimension, D, do not fall within the dimension, D, value range, transmitting a divert to waste command to divert the sample solution to a waste container.

A computer program product comprising a computer readable storage medium having program instructions embodied therewith, the program instructions executable by a processor to cause the processor to perform a method comprising:

• receiving from a multi-angle light scattering (MALS) instrument baseline scattering intensity values collected over a first period of time of a pure buffer flowed from a chromatography system to the MALS instrument;
• in response to receiving the baseline scattering intensity values of the pure buffer, receiving from the MALS instrument scattering intensity values collected over a second period of time of a sample solution flowed from the chromatography system to the MALS instrument, the second period of time being after the first period of time;
• and in response to receiving the scattering intensity values of the sample solution, characterizing at least one component of the sample solution, resulting in a real-time set of values of a dimension, D, of the at least one component and a real-time set of values of excess Rayleigh ratio, R0, of the at least one component,
• wherein the values of the dimension, D, correspond to one of a root-mean-square (rms) radius, Rg, of the at least one component, a sphere radius, R, of the at least one component, a rod length, L, of the at least one component, and a shape dimension relevant to a shape of the at least one component;
• determining that the values of the dimension, D, fall within a dimension, D, value range and that the values of excess Rayleigh ratio, R0, fall within an excess Rayleigh ratio, R0, value range,
• wherein values of the excess Rayleigh ratio, R0, within the excess Rayleigh ratio, R0, value range indicate that a sufficient signal has been received from the MALS instrument for a reliable calculation of the values of the dimension, D,
• in response to determining that the values of the dimension, D, fall within the dimension, D, value range, transmitting a collect sample solution command to collect the sample solution in a product pool,
• determining that at least one of the values of the dimension, D, do not fall within the dimension, D, value range, and
• in response to determining that at least one of the values of the dimension, D, do not fall within the dimension, D, value range transmitting a divert to waste command to divert the sample solution to a waste container.