Recombinant expression vector applicable to rapid screening for recombinant strain and application

A method for the rapidly screening Trichoderma reesei with enhanced expression or activity of cellulase including the steps of:

• (1) introducing a gene expression cassette comprising a cbh1 promoter, a red fluorescent protein gene DsRed, a cell surface protein anchor signal peptide gene AfMp1 and a cbh1 terminator, into the plasmid to obtain a recombinant expression vector, wherein said DsRed gene has the nucleotide sequence of SEQ ID No:
• 2, and said AfMp1 gene has the nucleotide sequence of SEQ ID No:
• 3;
• (2) transforming a Trichoderma reesei cell with the recombinant vector constructed in step (1) to obtain a recombinant Trichoderma reesei;
• (3) introducing a protein secretion pathway related gene or into the recombinant Trichoderma reesei obtained in step 2 to obtain a mutant library of the recombinant Trichoderma reesei;
• (4) screening of the recombinant Trichoderma reesei mutant library from part (4) and selecting those that show red fluorescence on its surface as determined by flow cytometry;
• and
• (5) determining the cellulase activity of the recombinant Trichoderma reesei obtained in step (4), to obtain the recombinant Trichoderma reesei with enhanced expression or activity of cellulase.