System and method for detecting a target enzyme

A system for detecting a target enzyme in a sample by way of an amplification loop, comprising:

• a substrate, a first enzyme comprising alginate lyase immobilized on the substrate via a first linker, and a second enzyme comprising collagenase immobilized on the substrate via a second linker;
• wherein the amplification loop is triggered by the target enzyme upon cleaving the first linker to release the first enzyme for cleaving the second linker, thereby releasing the second enzyme, and wherein the released second enzyme has a structure that allows it to cleave the first linker to release more of the first enzymes for continuously triggering the enzymatic cleavage reaction on the substrate.