Production of lipids and terpenoids in Auxenochlorella protothecoides
AssigneesPhycoil Biotechnology International, Inc., Phycoilbiotech Korea, Inc.
InventorsJeffrey Moseley, Byung-Hee Lee, Chung Soon Im, Jane Kim, Dayoung Kim, Riyaz Bhat
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Abstract
Methods to produce oils with modified profiles of fatty acid, carotenoids and/or terpenoids in microalgal mutants are provided. Microalgal mutants produce the oil containing fatty acids, carotenoids and/or terpenoids of a modified profile with a disruption or ablation of one or more alleles of an endogenous polynucleotide or comprising an exogeneous gene are also provided.
Claims12
A method for producing oils with modified profiles of omega-3 fatty acids, omega-6 fatty acids, lutein, zeaxanthin, astaxanthin, 4-keto-lutein, or squalene, comprising:
- growing a microalgal mutant, wherein the microalgal mutant is made by knock-out, knock-in or homologous recombination, wherein the microalgal mutant is a mutant of Auxenochlorella protothecoides, wherein the microalgal mutant comprises a knock-out of at least one allele of lycopene cyclase epsilon LCYE-1, lycopene cyclase epsilon LYCE-2, squalene epoxidase SQE-1, or squalene epoxidase SQE-2, or a replacement of a native FAD3 promoter by a FATA gene promoter from Auxenochorella protothecoides or a stearoyl-ACP desaturase (SAD2) promoter from Auxenochorella protothecoides, and
- isolating the oil from the microalgal mutant.
Dependencies are inferred from the claim text.