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US 12,037,611 B2Granted
Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
AssigneeR & D Systems, Inc.
InventorsDavid Largaespada, Branden Moriarity, Beau Webber, Neil Otto, Sandeep Kumar, Leah Hogdal
Life of this patent
Estimating the term…
Abstract
This disclosure provides various TcBuster transposases and transposons, systems, and methods of use.
Claims19
A mutant TcBuster transposase comprising an amino acid sequence at least 70% identical to full-length SEQ ID NO:
- 1 and having one or more amino acid substitutions that increase a net charge at a neutral pH in comparison to SEQ ID NO:
- 1, wherein the one or more amino acid substitutions comprise at least one of D99A, E247K, and E469K, when numbered in accordance with SEQ ID NO:
- 1;
- and
- wherein the mutant TcBuster transposase has increased transposition efficiency in comparison to a wild-type TcBuster transposase having the amino acid sequence SEQ ID NO:
- 1.
3 more elements
A mutant TcBuster transposase comprising an amino acid sequence at least 95% identical to full-length SEQ ID NO:
- 1 and comprising the following amino acid substitutions when numbered in accordance with SEQ ID NO:
- 1:
- N85S, D99A, E247K, V377T, and E469K.
Dependencies are inferred from the claim text.