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US 12,037,608 B2Granted
Genetically engineered coxsackievirus, and pharmaceutical composition
AssigneesThe University of Tokyo, N.P.T. S.R.L.
InventorsYang Jia, Shohei Miyamoto, Kenichiro Hara, Atsuhumi Iwanaga, Kenzaburo Tani
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Abstract
A gene-modified coxsackievirus that is to be used in oncolytic virotherapy and is improved in safety and/or aggressiveness is provided. A gene-modified coxsackievirus that contains a mutated genome with a coxsackievirus B3 wild-type (CVB3-WT) genome inserted with at least one polynucleotide constituted of a target sequence for at least one of either of miR-34a and miR-34c and is suppressed in proliferation in normal cells is provided.
Claims13
A gene-modified coxsackievirus comprising:
- a mutated genome including a coxsackievirus wild-type (CVB3-WT) genome, the CVB3-WT genome inserted with at least one polynucleotide wherein the polynucleotide includes a target sequence for a normal-cell-specific microRNA (miRNA),
- wherein proliferation of the gene-modified coxsackievirus is suppressed specifically in normal cells and
- wherein the target sequence for the normal-cell-specific miRNA corresponds to at least one of miR-34a and miR-34c, and
- a position of insertion of the polynucleotide includes at least a position between position 742 and position 743 inside a 5′ UTR region of the CVB3-WT genome,
- wherein the position of insertion of the polynucleotide corresponds to being immediately upstream a base sequence comprising an initiation codon that initiates the translation of the CVB3-WT genome into proteins.
2 more elements
Dependencies are inferred from the claim text.