Back to results
US 12,037,603 B2Granted
Methods for tissue fabrication
AssigneeOrganovo, Inc.
InventorsKelsey Nicole Retting, Deborah Lynn Greene Nguyen, Sharon C. Presnell, Shelby Marie King
Life of this patent
Estimating the term…
Abstract
Disclosed herein are improved methods for fabricating bioprinted, three-dimensional, biological tissues. The methods relate to exposures to low temperatures, incubations at low temperatures of various durations, and fabrication in environments without structural cross-linking treatments.
Claims17
A method of fabricating a three-dimensional, engineered, biological tissue, the method comprising:
- a) preparing a bio-ink comprising living cells, wherein the bio-ink is a viscous liquid, a semi-solid, or a solid;
- b) depositing the bio-ink onto a surface by extrusion bioprinting to form a structure;
- c) exposing the bio-ink to a hypothermic hold of greater than or equal to 2° C., but less than 10° C. after bioprinting;
- and
- d) incubating the bio-ink at a temperature of greater than or equal to 18° C., but less than 37° C. after the hypothermic hold;
- wherein the bio-ink is not exposed to any ionic, chemical, photo or physical cross-linker during the incubation at a temperature of greater than or equal to 18° C., but less than 37° C., and wherein the shape of the structure is maintained during the hypothermic hold and the incubating.
3 more elements
A method of fabricating a three-dimensional, engineered, biological tissue, the method comprising:
- a) preparing a plurality of bio-inks comprising living cells, wherein the bio-ink is a viscous liquid, a semi-solid, or a solid;
- b) depositing a first bio-ink onto a surface by extrusion bioprinting;
- c) incubating the first bio-ink at a temperature of greater than or equal to 18° C., but less than 37° C.;
- d) depositing a second bio-ink onto a surface by extrusion bioprinting;
- and
- e) incubating the plurality of bio-inks at a temperature of greater than or equal to 18° C., but less than 37° C.;
- further comprising:
- exposing the first bio-ink to a hypothermic hold of greater than or equal to 2° C., but less than 10° C. after bioprinting the first bio-ink and before incubating the first bio-ink, exposing the plurality of bio-inks to a hypothermic hold of greater than or equal to 2° C., but less than 10° C. after bioprinting the second bio-ink and before incubating the plurality of bio-inks, or both;
- wherein the first bio-ink, the plurality of bio-inks, or both are not exposed to any ionic, chemical, photo or physical cross-linker during the incubating at a temperature of greater than or equal to 18° C., but less than 37° C., wherein the depositing of the first bio-ink and the second bio-ink forms a structure, and wherein the shape of the structure is maintained during the hypothermic hold and the incubatings.
6 more elements
Dependencies are inferred from the claim text.