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US 12,037,578 B2Granted
Methods for isolating DNA from a blood sample
AssigneeBiocaptiva Limited
InventorsMark Bradley, Matthew Owens
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods for isolating nucleic acids present in a sample, in particular cell-free DNA (cfDNA) from a blood sample and polymers, substrates and kits for the method. Polymers with characteristics suitable to bind such nucleic acids are provided.
Claims18
A method for the isolation of nucleic acids from a sample, the method comprising the steps of:
- (a) providing the sample comprising nucleic acids to be isolated to a nucleobase containing polymer wherein the polymer further comprises other positively charged moieties at physiological pH adapted to electrostatically interact with the nucleic acids to be isolated, and/or adapted to interact with the nucleic acids to be isolated via hydrogen bonding, and
- (b) incubating at suitable conditions for binding the nucleic acids to be isolated to the polymer, followed by isolating the nucleic acids,
- comprising the following steps:
- washing a complex formed between the nucleic acids to be isolated and the polymer by a wash buffer to remove one or more other components of the sample from the polymer while retaining the nucleic acids to be isolated on the polymer;
- and
- eluting the nucleic acids to be isolated from the polymer by an elution buffer wherein the elution buffer has an ionic strength selected from equivalent to or higher than that of 600 mM NaCl solution and optionally the elution buffer has a pH of 5 or higher.
3 more elements
A method for the isolation of nucleic acids from a sample, the method comprising the steps of:
- (a) providing the sample comprising nucleic acids to be isolated to a nucleobase containing polymer wherein the polymer further comprises other positively charged moieties at physiological pH adapted to electrostatically interact with the nucleic acids to be isolated, and/or adapted to interact with the nucleic acids to be isolated via hydrogen bonding, and
- (b) incubating at suitable conditions for binding the nucleic acids to be isolated to the polymer, followed by isolating the nucleic acids,
- wherein the nucleobase-containing polymer comprises:
- (i) one or more monomers selected from the group consisting of 2-methoxyethyl acrylate, 2-methoxyethyl methacrylate, diethylamino ethylacrylate, diethylamino ethyl methacrylate, hydroxyethyl acrylate, hydroxyethyl methacrylate, methyl methacrylate, methyl acrylate, styrene, methyl styrene, glycidyl acrylate, glycidyl methacrylate, N-vinylacetamide, 2-methyl-2-nitropropyl methacrylate, acrylic acid, methacrylic acid, 2-[[(butylamino)carbonyl]oxy] ethylacrylate, dimethylamino ethyl acrylate, dimethylamino ethyl methacrylate, mono-2-(acryloyloxy)ethyl succinate, poly(ethylene glycol) methyl ether acrylate/methacrylate and combinations thereof, and (ii) one or more (meth)acrylate-based monomers with nucleobase side chains.
1 more element
Dependencies are inferred from the claim text.